Home Faculty Education Research Achievements Seminars & Events
Administration Contact

Jugsharan Singh Virdi

Professor
Department of Microbiology
Bachhawat Block, First Floor
University of Delhi South Campus

Ph: 011-24157164
Email: virdi_dusc@rediffmail.com 
CV (Download pdf file)
  Education
BSc (1975), Panjab University, Chandigarh
MSc (1977), Panjab University, Chandigarh
PhD (1986), PGI Chandigarh
 
Description of Research Expertise

Research interest: Molecular epidemiology of emerging food- and water-borne pathogens, Antimicrobial Resistance (AMR) and its rapid detection

Key words: Yersinia enterocolitica, Antimicrobial Resistance (AMR), β-lactamases, POC detection of antimicrobial resistance

Description of Research: The focus of the lab has been on emerging food- and water-borne pathogens especially molecular epidemiology of Yersinia enterocolitica and antibiotic resistant E.coli, and their public health significance in India. Studies on molecular epidemiology of Yersinia enterocolitica revealed that though several serotypes were present in India, genotypically these constituted only two clonal groups. The majority of the Indian strains though biovar 1A are enterotoxigenic. Comparative genomic studies using VNTRs, minimum spanning tree, MLRT, & e-BURST showed that clinical biovar 1A strains originated from aquatic strains by genetic change and host adaptation. The study also revealed that Indian strains possessed unique β-lactamases. The sequencing of β-lactamase genes (bla) and their flanking regions in Y.enterocolitica and E.coli, and their comparison with diverse bla genes in databases suggested that these may serve as a goldmine for rapid detection of antibiotic resistance in different bacteria.  Building on these leads from the work carried out in the laboratory, the lab is currently engaged in developing a rapid test in point of care (POC) format for detection of antibiotic resistance using the state of art microbial genomics (bacterial whole genome sequencing, resistome analysis) and informatics approach in collaboration with bioinformaticians and inputs from clinical microbiologists and microfluidics experts. This idea was adjudged best and awarded the first prize by BIRAC-DBT-NESTA for development. This work is currently being carried out in the laboratory further to develop the test for rapid detection of antibiotic resistance in bacteria.
 

Lab Personnel

              CURRENT

                    ALUMUS NAME (Work Place)

Dr. Neelja Singhal (DST)

Dr. Indrajit Sinha (Acenzia Inc. Ontario, Canada)

Dr. Jyotsana Dalal (UGC)

Dr. Itender Singh (Washington University)

Dr. Pawan K.  Kanaujia (ICMR)

Dr. Pooja Gulati (Maharshi Dayanand Uni, Rohtak)

Somendro N. Singh (ICMR)

Dr. Sachin Sharma (Wellcometrust DBT IA)

Rajesh K. Mahto (DST-PURSE)

Dr. Sarita Mallik (Univ of Bloomington, USA)

Dr. Neeru Bhagat ( formerly at IHE, DU)

Dr. Pradeep Kumar

Dr. Mahesh S. Dhar (Amity Uni, NOIDA)

Dr. Asani Bhaduri (Cluster Innovation Centre, DU)

Dr. Pawan K. Kanaujia (Univ Delhi South Campus)

Dr. Priyanka Bajaj ( Centre for Molecular Medicine, JNU)

Distinctions:

                                   

                                     

Clockwise: Yersinia enterocolitica work from lab appears on cover page of Current Science (2000); Prof. J.S.Virdi conferred ICMR Y.S. Narayanarao Award for research in Microbiology (2006); UDSC-AMR research group won First Ideathon Award by DBT-BIRAC-NESTA (2016); Prof. Virdi nominated as one of the three finalists for ThermoFischer Invitrogen Best Lab Mentor Award (2016)

Publications:

1.     Bajaj, P., Singh, N.S., & Virdi, J.S. (2016). Escherichia coli β-Lactamases: What Really Matters.

Front Microbiol. 2016 Mar 30;7: 417. doi: 10.3389/fmicb.2016.00417. 

2.     Singhal, N., Kumar, M., Kanaujia, P.K., & Virdi, J.S. (2015). MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis. Front Microbiol. Aug 5;6:791.

3.     Singhal, N., Srivastava, A., Kumar, M., & Virdi, J.S. (2015). Structural variabilities in β-lactamase (blaA) of different biovars of Yersinia enterocolitica: Implications for β-lactam antibiotic and β-lactamase inhibitor susceptibilities. PLoS One 10(4):e0123564. 

4.     Kanaujia, P.K., Bajaj, P., Kumar, S., Singhal, N., & Virdi, J.S. (2015). Proteomic analysis of Yersinia enterocolitica biovar 1A under iron-rich and iron-poor conditions indicate existence of efficiently regulated mechanisms of iron homeostasis. J Proteomics, 124: 39-49. 

5.     Srivastava, A., Singhal, N., Goel, M., Virdi, J.S., & Kumar, M. (2014). CBMAR: a comprehensive β-lactamase molecular annotation resource. Database (Oxford) 2014:bau111. 

6.     Singhal, N., Kumar, M., & Virdi, J.S. (2014). Molecular analysis of β-lactamase genes to understand their differential expression in Y. enterocolitica biotype 1A.Sci Rep. 4:5270. 

7.     Singh, D., Sharma, K.K., Dhar, M.S., & Virdi, J.S. (2014) Molecular modeling and docking of novel laccase from multiple serotype of Yersinia enterocolitica suggests differential and multiple substrate binding. Biochem Biophys Res Commun. 449(1)157-162. 

8.     Rastogi, N., Nagpal, N., Alam, H., Pandey, S., Gautam, L., Sinha, M., Shina, K., Manzoor, N., Virdi, J.S., Kaur, P., Sharma, S., & Singh, T.P. (2014). Preparation and antimicrobial action of three tryptic digested functional molecules of bovine lactoferrin. PLoS One 9 (3) e90011. 

9.     Bhaduri, A., Misra, R., Bhetaria, P., Mishra, S., Maji, A., Arora, G., Virdi, J.S., & Singh, Y. (2013). Mycobacterium tuberculosis cyclophilin A uses novel signal sequence for secretion and mimics eukaryotic cyclophilins for interaction with host protein repertoire PLoS One 9 (3) e88090 

10. Dhar, M.S., Gupta, V., & Virdi, J.S. (2013). Detection, distribution and characterization of novel superoxide dismutases from Yersinia enterocolitica Biovar 1A. PLoS One, 8, e63919. 

11. Bhagat, N., & Virdi, J.S. (2011). The enigma of Yersinia enterocolitica biovar 1A. Crit Rev Microbiol, 37(1), 25-39. 

12. Mallik, S., & Virdi, J.S. (2010). Genetic relationships between clinical and non-clinical strains of Yersinia enterocolitica biovar 1A as revealed by multilocus enzyme electrophoresis and multilocus restriction typing. BMC Microbiology, 28, 10:158. 

13. Bhagat, N., & Virdi, J.S. (2009). Molecular and biochemical characterization of urease and survival of Yersinia enterocolitica biovar 1A in acidic pH in vitro. BMC Microbiol, 9, 262. 

14. Mittal S, Mallik S, Sharma S, Virdi JS (2007) Characteristics of β-lactamases and their genes (bla A and bla B) in Yersinia intermedia and Y. frederiksenii. BMC Microbiology, 7: 25. 

15. Bhagat N and Virdi JS (2007) Distribution of virulence-associated genes in Yersinia enterocolitica biovar 1A correlate with clonal groups and not the source of isolation. FEMS Microbiology Letters 266: 177-186. 

16. Sharma S, Mittal S, Mallik S and Virdi JS (2006) Molecular characterization of beta-lactamase genes blaA and blaB of Yersinia enterocolitica biovar 1A. FEMS Microbiology Letters 257: 319–327. 

17. Sachdeva P, Virdi JS (2004) Repetitive elements sequence (REP/ERIC) - PCR based genotyping of clinical and environmental strains of Yersinia enterocolitica biotype 1A reveal existence of limited number of clonal groups. FEMS Microbiology Letters, 240:193-201. 

18. Sharma S, Ramnani P, Virdi JS (2004) Detection and assay of b–lactamases in clinical and non-clinical strains of Yersinia enterocolitica biovar 1A. J. Antimicrobial Chemotherapy, 54:401-405. 

Search Pubmed for publications (click here)

*Website content created & maintained by Department of Microbiology, University of Delhi South Campus