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Apr 23, 2018
Category: General
Posted by: admin

The Department of Microbiology is in the process of revising the syllabus of M.Sc. Microbiology program under Choice Based Credit System. The draft of the revised syllabus has been prepared after taking into consideration the feedback obtained from multiple stakeholders including the present students, the alumni, as well as prospective employers from the corporate sector. The Department invites your views and suggestions on the same which may be submitted only by email at microsdc@south.du.ac.in latest by 5 PM on Friday, the 4th of May 2018. The draft of syllabus may be downloaded here.

Apr 17, 2018
Category: General
Posted by: admin

On 20-April-2018, Ms.Nivedita Gaur will present her pre-PhD submission seminar entitled -“The role of human tumor associated herpesvirus latent antigens in epithelial to mesenchymal transition of cancer cells" at 11AM in Seminar room of the Department. 

Apr 13, 2018
Category: General
Posted by: admin

On 13-Apr-2018, Ms.Ashima Dua will be presenting a paper which may be downloaded by clicking on the image below. This will be followed by work presentation by Mr.Gaurav Kumar.

Paper Cover

CMS - 2.2.7 - Skookumchuck
 

Publications

Dr.Rani Gupta

Lab Members

Publications

Projects

Sequence Submissions

Alumni

Gallery

​From 2012-2017:

2017:

Bindal, S., & Gupta, R. (2017). Hyperproduction of gamma-glutamyl transpeptidase from Bacillus licheniformis ER15 in the presence of high salt concentration. Preparative Biochemistry and Biotechnology, 47(2), 163-172.

Bindal, S., Sharma, S., Singh, T. P., & Gupta, R. (2017). Evolving transpeptidase and hydrolytic variants of gamma-glutamyl transpeptidase from Bacillus licheniformis by targeted mutations of conserved residue Arg109 and their biotechnological relevance. J of Biotechnology, 249, 82-90.

Dua, A., & Gupta, R. (2017). Functional characterization of hormone sensitive-like lipase from Bacillus halodurans: synthesis and recovery of pNP-laurate with high yields. Extremophiles, 21(5), 871-889.

Syal P., Verma V V., & Gupta, R. (2017). Targeted mutations and MD simulations of a methanol-stable lipase YLIP9 from Yarrowia lipolytica MSR80 to develop a biodiesel enzyme. IntJ Biol Macromo, 104, 78-88.

 

2016:

Bindal S and Gupta, R. (2016). Thermo- and salt-tolerant chitosan cross-linked γ-glutamyl transpeptidase from Bacillus licheniformis ER15. IntJ Biol Macromol. 91, 544-553.

Saini, M, Bindal, S and Gupta, R. (2016). Heterologous expression of γ-glutamyl transpeptidase from Bacillus atrophaeus GS-16 and its application in the synthesis of γ-D-glutamyl-L-tryptophan, a known immunomodulatory peptide. Enzyme Microb. Technol, doi.org/10.1016/j.enzmictec.2017.01.003.

Singh Y, Gupta N, Verma VV, Goel M, and Gupta, R. (2016). Selective disruption of disulphide bonds lowered activation energy and improved catalytic efficiency in TALipB from Trichosporon asahii MSR54: MD simulations revealed flexible lid and extended substrate binding area in the mutant. Biochem Biophys Res Commun, 472, 223-30.

Syal, P. and Gupta, R. (2016). Heterologous expression of lipases YLIP4, YLIP5, YLIP7, YLIP13 and YLIP15 from Yarrowia lipolytica MSR80 in E. coli: Substrate specificity, kinetic comparison and enantioselectivity. Biotech Appl Biochem, doi:10.1002/bab.1542.

 

2015:

Arti, A, Baronian, K, Kunze, G, Gupta, R. (2015). Extracellular expression of YlLip11 with a native signal peptide from Yarrowia lipolytica MSR80 in three different yeast hosts, Protein Express Purif, 110:138-144.

Gupta, R, Kumari, A, Syal, P, Singh, Y (2015) Molecular and functional diversity of yeast and fungal lipases: Their role in biotechnology and cellular physiology. Prog Lipid Research, 57: 40-54.

Kumari, A, Gupta, R. (2015) Functional characterization of a novel aspartic acid rich lipase, TALipC, from Trichosporon asahii MSR54: solvent-dependent enantio inversion during esterification of 1-phenylethanol, Biotechnol Lett, 37(1):121-30.

Singh, Y, Gupta, R. (2015). Novel S-enantioselective lipase TALipB from Trichosporon asahii MSR54: Heterologous expression, characterization, conformational stability and homology modeling. Enzyme and Microb.l Technol., 83, 29–39

 

2014:

Bindal, S., Gupta, R. (2014). L-Theanine synthesis using γ-glutamyl transpeptidase from Bacillus licheniformis ER-15. J Agric Food Chem, 62 (37), 9151–9159.

Kumari, A., Gupta, R. (2014). Functional characterization of a novel aspartic acid rich lipase, TALipC, from Trichosporon asahii MSR54: solvent-dependent enantio inversion during esterification of 1-phenylethanol. Biotechnol Lett, doi 10.1007/s10529-014-1648-5

Kumari, A., Gupta, R. (2014). Functional characterisation of novel enantioselective lipase TALipA from Trichosporon asahii sequence comparison revealed new signature sequence AXSXG among yeast lipase. Appl Biochem Biotechnol, doi 10.1007/s12010-014-1268-5

Kumari, A., Gupta, R. (2014). Novel strategy of using methyl esters as slow release methanol source during lipase expression by mut+ Pichia pastoris X33. PLoS ONE 9(8): e104272. doi:10.1371/journal.pone.0104272

 

2013:

Gupta R, Sharma R and Beg Q (2013), Revisiting microbial keratinases: Next generation proteases for sustainable biotechnology, Crit Rev Biotechnol, 33(2) 216-228

Kumari, A., Gupta, R. (2013), Heterologous expression, purification and characterization of thermostable lip11 from Yarrowia lipolytica in Pichia pastoris X33, J Prot Proteomics, 4, 5-10.

Rajput, R, Gupta, R. (2013), Expression of Bacillus pumilus keratinase rK27 in Bacillus subtilis: enzyme application on developing renewable flocculants from bone meal, Ann Microbiol, DOI 10.1007/s13213-013-0770-2

Rajput, R, Gupta, R. (2013), Thermostable keratinase from Bacillus pumilus KS12: Production, chitin crosslinking and degradation of Sup35NM aggregates, Bioresource Technol, 133, 118-126.

Rajput R, Verma VV, Chaudhary V and Gupta R (2013), A hydrolytic γ-glutamyltranspeptidase from thermo-acidophilic archaeon Picrophilus torridus: binding pocket mutagenesis and transpeptidation, Extremophiles, 17, 29-41

Rajput R, Verma VV, Chaudhary V, Gupta R. (2013) A hydrolytic γ-glutamyl transpeptidase from thermo-acidophilic archaeon Picrophilus torridus: binding pocket mutagenesis and transpeptidation. Extremophiles, 17(1):29-41

Rajput R, Gupta R. (2013) Thermostable keratinase from Bacillus pumilus KS12: Production, chitin crosslinking and degradation of Sup35NM aggregates. Bioresour Technol., 133C:118-126.

Sharma R, Verma VV and Gupta R (2013), Functional characterization of an extracellular keratinolytic protease, Ker AP from Pseudomonas aeruginosa KS-1: A putative aminopeptidase with PA domain, J. Mol. Catal., 91, 8-16

 

2012:

Kumari A, Gupta R. (2012) Extracellular expression and characterization of thermostable lipases, LIP8, LIP14 and LIP18, from Yarrowia lipolytica. Biotechnol Lett., 34(9):1733-9.

Murty NA, Tiwary E, Sharma R, Nair N, Gupta R. (2012) γ-Glutamyl transpeptidase from Bacillus pumilus KS 12: decoupling autoprocessing from catalysis and molecular characterization of N-terminal region. Enzyme Microb Technol., 50(3):159-64

Rajput R, Tiwary E, Sharma R, Gupta R. (2012). Swapping of pro-sequences between keratinases of Bacillus licheniformis and Bacillus pumilus: altered substrate specificity and thermostability. Enzyme Microb Technol.,51(3):131-135.