Prof. Yogender P. Khasa
Professor
email: yogi110@gmail.com; ypkhasa@south.du.ac.in
Phone: 011-24157369
Area of research: Bioprocess Engineering and Industrial Biotechnology
Research description:
Our laboratory is focused on the large scale production of various proteins of therapeutic importance for clinical applications. The genes encoding asparaginase, scFvs, hTNF-α, hG-CSF, hGM-CSF, hIL-3, hIL-7 and streptokinase have been cloned and expressed in two heterologous systems: E. coli and Pichia pastoris. Expression studies using different signal sequences and fusion partners resulted in the soluble expression of these proteins. Expression in Pichia permitted scale- up in a 5 l bioreactor under optimized parameters that helped in achieving upto 300 g/L cell biomass (WCW). The large scale recovery of these biomolecules has been established with their subsequent testing for biological activity using cell culture experiments. The indigenous production process of ULP1 protease has been successfully developed to reduce the overall manufacturing cost of recombinant proteins in soluble form. The bioprocess optimization of therapeutically important recombinant proteins such as streptokinase, hIL-3 and hIL-7 was carried out using the 5l bioreactor, where gram level production of these molecules has been obtained. As long-term goal, we are working towards a comprehensive technology development for these proteins for commercial applications.
Awards:
2010 : Dr. Y. P. Khasa: Young Scientist Award in “Molecular Microbiology” 2010 by Association of Microbiologists of India (AMI).
Memberships of Professional Bodies/ Associations:
Life member of “Association of Microbiologists of India (AMI)” of Delhi Chapter of AMI (No. 1119 – 2010)
Life Member “The Biotech Research Society, India” (LM 1768 )
Invited Guest Lecture:
Invited guest lecture on “Bioprocess optimization of recombinant therapeutics in methylotrophic yeast P. pastoris: Streptokinase as a model protein” in 58th Annual conference of AMI titled “Microbes for Sustainable Development: Scope & Applications” at Babashaheb Bhimrao Ambedkar University (A Central University), Lucknow, Uttar Pradesh, India between November 16-19, 2017.
Invited guest lecture on “Bioprocess development for the over-production of recombinant Streptokinase in Pichia pastoris” in National conference on Biotechnology: Exploring through Innovations (BETI)-2017" at Central University of Haryana, Mahendergarh, Haryana, between November 13-14, 2017.
Invited guest lecture on “High level production of recombinant streptokinase in Pichia pastoris fed-batch fermentation” in International conference on Translational biotechnology “BioSangam 2016” between 4-6th Febrauary 2016, at Motilal Nehru National Institute of Technology (MNNIT) Allahabad- 211004 UP, India.
Invited guest Lecture on “Extracellular production of recombinant Streptokinase in Pichia pastoris” in National seminar on “Recent advances in Agriculture, Biomedical & Environmental Biotechnology” between May 01-02, 2015 at Anand Engineering College, Agra.
Invited guest Lecture on “High level expression of active recombinant streptokinase in Pichia pastoris” in National conference on Evolving trends in Biotechnology between March 28-30, 2015 at Dr. Harisingh Gour Central University, Sagar, MP-470003.
Invited guest Lecture on “Recombinant therapeutics and their expression bottlenecks” in Indo-Italian workshop on food technology and cold chain management” at Amity University, Noida, UP on 26th-27th November, 2014
Invited guest Lecture on “Recent advancements in bioprocess technology: a case study of recombinant therapeutics expression in E. coli” in National conference on Bioprocess Technology: Basics, Advancements and Challenges (BTBAC 2014) on September 13th 2014 at Jaipur National University, Jaipur, Rajasthan-302017
Invited guest Lecture on “Expression of recombinant therapeutics in Pichia pastoris: Human Interleukin-3 (hIL-3) as a model system” in International conference on “Frontier discoveries and emerging opportunities in life Sciences” between February 13-15, 2014 at Dr. Harisingh Gour Central University, Sagar, MP-470003.
Invited guest Lecture on “Therapeutic Proteins: Expression and their optimization bottlenecks” at Department of Microbiology, Punjab University Chandigarh on 20th Sept. 2013.
Invited guest Lecture at training program on “Application of Modern Biotechnological Techniques in Molecular Biology Research” at Indian Veterinary Research Institute (IVRI), Mukteswar, Uttarakhand sponsored by Uttarakhand State Biotechnology Department (USBD) from December 3rd to 17th, 2012.
Invited lecture in National Science day symposium titled “Recombinant protein expression and optimization strategies” on 28th Feb. 2011 at University of Delhi South campus, New Delhi India.
Invited Lecture in Nation Conference on Microbial Biotechnology “Microcon 2011: Microbes in the service of society” at Punjab University Chandigarh, January 11-12, 2011.
Invited Young Scientist Lecture in 51st Annual Conference “International Symposium on Recent Advances in Cross-disciplinary Microbiology: Avenues and Challenges & International Workshop on rRNA Sequencing, Phylogeny & Next Generation Genome Sequencing” organized by Association of Microbiologists of India at Birla Institute of Technology (BIT), Mesra, Ranchi December 14-17 2010.
Select Publications:
Dagar VK, Adivitiya, and Khasa YP (2018) “Combined effect of gene dosage and process optimization strategies on high-level production of recombinant human Interleukin-3 (hIL-3) in Pichia pastoris fed-batch culture” International Journal of Biological Macromolecules, 108:999-1009.
Adivitiya, and Khasa YP (2017) “The evolution of recombinant thrombolytics: Current status and future directions.” Bioengineered 8(4): 331-358.
Dagar, V. K., Adivitiya, Devi, N., and Khasa, Y. P. (2016). Bioprocess development for extracellular production of recombinant human interleukin-3 (hIL-3) in Pichia pastoris. Journal of Indusrial Microbiology and Biotechnology, 43 (10):1373-1386.
Devi, N., Adivitiya, and Khasa, Y. P. (2016). A combinatorial approach of N-terminus blocking and codon optimization strategies to enhance the soluble expression of recombinant hIL-7 in E. coli fed-batch culture. Applied Microbiology and Biotechnology, 100 (23): 9979-9994.
Adivitiya, Dagar, V. K., Devi, N., and Khasa, Y. P. (2016). High level production of active streptokinase in Pichia pastoris fed-batch culture. International Journal of Biological Macromolecules, 83: 50-60.